Comparison of Lipase Activities by Different Assays

Abstract

Recently several quick assay procedures have been developed (1 to 15), and are saidto detect lipase activity. The hydrolysis of Triglycerols and artificial substrates oftencan not explain differences of whatis a lipase and whatis an esterase. We established a numberoflipase assays for the estimation ofactivities for a large numberof different lipase preparations, as immobilized pure enzymes, nearly homogeneous preparations and samples of industrial grade. We found remarkable differences concerning the lipase activities measured with different assays and report about characteristics and comparability. At last, we want to arise the question "do we look for the enzymes we are interested in ?"