ENZYME ELECTRODES FOR MEDICAL APPLICATIONS

Abstract

1.The development and optimization of a highly sensitive creatine sensor based on the creatine amidinohydrolase - sarcosine oxidase sequence is described. The bi-enzyme sensor for the analysis of creatinine and creatine kinase is characterized by the following analytical performance: response time below 15 s, detection limit 5 uM creatine, linear concentration range up to 500 uM creatine, functional stability more than & days, serial imprecision below 5 %, measuring range for CK between 1 and 1000 U/l. 2. A well designed modified lactate sensor based on a cellulose-lactate oxidase/polycarbonate-cellulose membrane system is demonstrated for the accurate analysis of lactate in a concentration range between 0.2 and 20 mM using undiluted whole blood. This sensor now is commercialized as the main part of the BIOSENLactat2000 (EKF Industrie-Elektronik Magdeburg, Germany).