USE OF BIOSENSORS IN ANALYZERS FOR CLINICAL-CHEMICAL LABORATORIES

Abstract

Prüfgeräte-Werk Medingen GmbH is a middle class enterprise resident in the Dresden-Freital region. Since 1968, it has been manufacturing analyzers using the flow stream principle. Experience gathered during that period brought about in cooperation with Zentralinstitut für Molekularbiologie Berlin the application of the flow stream principle aiso for measurements using biosensors. A result are micro flow cells for determination of glucose or lactate in blood, serum or plasma which nowadays are being used in about 1.000 analyzers of the types ECA 20 and ESAT 6660. The measurement is based on detection of H202 being produced by the known reactions GOD 8-D-glucose + 02 + H20 —™ D-gluconic acid + H202 LOD L-lactäte#403%+ 238230) —e pyruvateii+'H303. Thus, a signal is obtained that is independent of the oxygen content in the blood. Easy handling, an optimized automatic regime of measurement and the availability of preconfectionated membranes and solutions are some reasons for the broad application.