FIRST STUDIES ON THE DEVELOPMENT OF A BIOSENSOR FOR TRIGLYCERIDES

Abstract

A new method for triglyceride analysis is described. It is based on the fluorescence measurement of NADH, which is obtained in a coupled enzymic Process, i.e., hydrolysis of triglycerides by regiounspecific lipase and subsequent oxidation of glycerol by NAD+ dependent glycerol dehydrogenase. The emerging fluorescence signal at 465 nm following excitation at 365 nm could be monitored also in turbic solutions, thus time consuming pretreatments such as extractions become obsolete. The method was also applied in a FIA-type arrangement.