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dc.contributor.authorSchmidt, H.-L.
dc.contributor.authorSchuhmann, W.
dc.contributor.authorMedina, R.
dc.contributor.authorUhe, B.
dc.contributor.authorHämmerle, M.
dc.contributor.authorGutberlet, F.
dc.date.accessioned2024-04-10T09:43:10Z
dc.date.available2024-04-10T09:43:10Z
dc.date.issued1992
dc.date.submitted2024-04-10
dc.identifier.citationBiosensors : fundamentals, technologies and applications, 97 - 105en_US
dc.identifier.isbn3527284370
dc.identifier.isbn1560812206
dc.identifier.issn0930-4320
dc.identifier.urihttp://hdl.handle.net/10033/623774
dc.description.abstractMost amperometric enzyme electrodes demand integrated coenzymes and/or mediators for the transfer of redox equivalents between protein and transducer. Thefree diffusibility required for this function of the mediators implies simultaneously their leaking from the electrode chamber. Approaches to overcomethis problem, aiming on the construction of compact independent oxidoreductase electrodes are reportedin this contribution. first attempt is based on the covalent binding of oxidoreductases on various conducting supports. Independent from the material and the binding method no direct electron transfer was obtained in any case. A subsequent immobilization of mediators through long spacersis therefore envisaged. In another approach, the functionalization of conducting surfaces with orientated mediators boundthrough conducting spacersis investigated, aiming on the subsequentaffinant binding of coenzymes. The most successful and promising way seemsto bind mediators or coenzymes through spacers to the enzymes themselves and to immobilize these complexes onto or within functionalized electrode surfaces.en_US
dc.language.isoenen_US
dc.publisherGBF Gesellschaft für Biotechnologische Forschung mbH, Braunschweigen_US
dc.relation.ispartofseriesGBF monographs ; Volume 17en_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleBIOSENSORS WITH OXIDOREDUCTASES AND INTEGRATED COENZYME OR MEDIATOR RECYCLINGen_US
dc.typeBook chapteren_US
dc.typeconference paperen_US
dc.contributor.departmentLehrstuhl für Allgemeine Chemie und Biochemie Technische Universität München D-8050 Freising-Weihenstephanen_US
dc.identifier.journalBiosensors : fundamentals, technologies and applications, 1992en_US
refterms.dateFOA2024-04-10T09:43:12Z


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Attribution-NonCommercial-ShareAlike 4.0 International
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