DIRECT OBSERVATION OF ANTI-ATRAZINE ANTIBODY BINDING USING GRATING COUPLERS

Abstract

Planar monomode waveguides with grating couplers have beenapplied for the direct detection of anti-atrazine antibodies and a pesticide assay has been developed on the basis of a competitive immunoassay. Data are shown for the binding of anti-atrazine antibodiesin the concentration range from 1 to 10 ug/ml to an immobilized atrazine-derivative, corresponding to a changein the adlayer thickness of between 0.1 and 3 A. First competition experiments using terbutryn showed significant suppression of antibody binding at a concentration of 10 ng/ml.