PREPARATION OF A NADH OXIDASE FOR BIOSENSOR APPLICATIONS

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Authors
Kondruweit, S.
Erdmann, Helmut
Park, H.-J.
Reiser, C. O. A.
Sprinzi, M.
Schmid, Rolf D.
Issue Date
1992
Submitted date
2024-04-17

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Abstract
A NADHoxidase from Thermus thermophilus HB8 was purified to homogeneity by a procedure that is easy to scale up. The hydrogen peroxide forming NADH oxidase was found to be a monomerof 26 kD by SDSPAGE, oxidation of NADH (NADPH) occuredin the presence of O, and either FMN or FAD. These cofactors also enhancedthe stability of the enzyme towards higher temperatures and extreme pH. The properties of the NADH oxidase are discussed in respectofits applicability in biosensor techniques.
Citation
Biosensors : fundamentals, technologies and applications, 245 - 250
Affiliation
GBF, Gesellschaft fur Biotechnologische Forschung mbH, Mascheroder Weg1, W-3300 Braunschweig, F.R.G.; Laboratorium für Biochemie, Universität Bayreuth, Universitätsstr. 30, W-8580 Bayreuth, F. R. G.
Publisher
GBF Gesellschaft für Biotechnologische Forschung mbH, Braunschweig
Journal
Biosensors : fundamentals, technologies and applications, 1992
URI
http://hdl.handle.net/10033/623798
Type
Book chapter
conference paper
Language
en
Series/Report no.
GBF monographs ; Volume 17
ISSN
0930-4320
ISBN
3527284370
1560812206
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