APPLICATION OF NADH OXIDASEIN FIBRE OPTIC BIOSENSORS

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Authors
Dremel, Bernd A. A.
Kondruweit, Simone
Erdmann, Helmut
Schmid, Rolf D.
Issue Date
1992
Submitted date
2024-04-17

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Abstract
A newfully reversible fibre-optic detection system based on the detection of NADH-fluorescence is presented. NADH oxidase (EC: 1.6.99.3) was used to regenerate NADH thatis needed for the oxidizing reaction of alcohols and aldehyds by different dehydrogenases.In the oxidation reaction NAD* wasreduced to NADH and the increase of fluorescence was monitored bya fibre-optic detection system. The NADH-fluorescence decreased in the absence of substrate due to the oxidation of NADH by NADH oxidase. Different types of NADH oxidase (Thermus thermophilus, Thermus aquaticus und Bacillus licheniformis) were studied in respectto their application in optical sensors. Only NADH oxidaseof B. licheniformis proved to beactive and stable at any assay conditions even in the absenceof FAD.
Citation
Biosensors : fundamentals, technologies and applications, 255 - 258
Affiliation
GBF, Gesellschaft fur Blotechnologische Forschung mbH, Department of Enzyme Technology, Mascheroder Weg 1, W-3300 Braunschweig, FRG
Publisher
GBF Gesellschaft für Biotechnologische Forschung mbH, Braunschweig
Journal
Biosensors : fundamentals, technologies and applications, 1992
URI
http://hdl.handle.net/10033/623800
Type
Book chapter
conference paper
Language
en
Series/Report no.
GBF monographs ; Volume 17
ISSN
0930-4320
ISBN
3527284370
1560812206
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