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APPLICATION OF NADH OXIDASEIN FIBRE OPTIC BIOSENSORS

dc.contributor.authorDremel, Bernd A. A.
dc.contributor.authorKondruweit, Simone
dc.contributor.authorErdmann, Helmut
dc.contributor.authorSchmid, Rolf D.
dc.date.accessioned2024-04-17T10:06:19Z
dc.date.available2024-04-17T10:06:19Z
dc.date.issued1992
dc.date.submitted2024-04-17
dc.identifier.citationBiosensors : fundamentals, technologies and applications, 255 - 258en_US
dc.identifier.isbn3527284370
dc.identifier.isbn1560812206
dc.identifier.issn0930-4320
dc.identifier.urihttp://hdl.handle.net/10033/623800
dc.description.abstractA newfully reversible fibre-optic detection system based on the detection of NADH-fluorescence is presented. NADH oxidase (EC: 1.6.99.3) was used to regenerate NADH thatis needed for the oxidizing reaction of alcohols and aldehyds by different dehydrogenases.In the oxidation reaction NAD* wasreduced to NADH and the increase of fluorescence was monitored bya fibre-optic detection system. The NADH-fluorescence decreased in the absence of substrate due to the oxidation of NADH by NADH oxidase. Different types of NADH oxidase (Thermus thermophilus, Thermus aquaticus und Bacillus licheniformis) were studied in respectto their application in optical sensors. Only NADH oxidaseof B. licheniformis proved to beactive and stable at any assay conditions even in the absenceof FAD.en_US
dc.language.isoenen_US
dc.publisherGBF Gesellschaft für Biotechnologische Forschung mbH, Braunschweigen_US
dc.relation.ispartofseriesGBF monographs ; Volume 17en_US
dc.rightsAttribution-NonCommercial-ShareAlike 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.titleAPPLICATION OF NADH OXIDASEIN FIBRE OPTIC BIOSENSORSen_US
dc.typeBook chapteren_US
dc.typeconference paperen_US
dc.contributor.departmentGBF, Gesellschaft fur Blotechnologische Forschung mbH, Department of Enzyme Technology, Mascheroder Weg 1, W-3300 Braunschweig, FRGen_US
dc.identifier.journalBiosensors : fundamentals, technologies and applications, 1992en_US
refterms.dateFOA2024-04-17T10:06:20Z


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Attribution-NonCommercial-ShareAlike 4.0 International
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