INVESTIGATIONS ON GLUCOSE BIOSENSORS USING LANGMUIR-BLODGETT TECHNIQUESFOR THE IMMOBILIZATION OF DERIVATIZED GLUCOSE OXIDASE

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Authors
Dremel, Bernd A. A.
Kalisz, Henryk M.
Schaffar, B. P. H.
Draxler, S.
Lippitsch, M. E.
Schmid, Rolf D.
Wolfbeis, Otto S.
Issue Date
1992
Submitted date
2024-04-17

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Abstract
Anovel method for the detection of glucose using modified glucose oxidase on Langmuir-Blodgett films is presented. A new polymer Langmuir-Blodgett film was used to immobilize modified glucose oxidase together with an oxygen sensitive fluorescent indicator. Five different glucose oxidase preparations were investigated: native, degtycosylated, N-palmitoy!-modified, periodate oxidized and y-carbodiimide treated. The oxygen consumption in the prescence of glucose was measured via dynamic quenchingof the fluorescence of the oxygen sensitive indicator.
Citation
Biosensors : fundamentals, technologies and applications, 259 - 262
Affiliation
GBF, Gesellschaft fur Biotechnologische Forschung, Mascheroder Weg 1, W - 3300 Braunschwelg, FRG; AVL List GmbH,Klelststr. 48, A-8020 Graz, Austria; Karl-Franzens Universitat, Heinrich Str. 28, A-8010 Graz, Austria
Publisher
GBF Gesellschaft für Biotechnologische Forschung mbH, Braunschweig
Journal
Biosensors : fundamentals, technologies and applications, 1992
URI
http://hdl.handle.net/10033/623801
Type
Book chapter
conference paper
Language
en
Series/Report no.
GBF monographs ; Volume 17
ISSN
0930-4320
ISBN
3527284370
1560812206
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