A COULOMETRIC SENSOR - ACTUATOR-SYSTEM FOR ENZYMATIC MEASUREMENTS WITH LONG TERM STABILITY

Abstract

Rapid and precise working microcoulometric flow titrations are suitable to follow enzyme catalyzed reactions producing ammonia. Ammonia was separated from the effluent of enzyme microreactors or directly from the enzyme immobilisate by an almost quantitatively working gas dialysis step. Highly precise and accurate determinations of urea, glutamine, asparagine and ammonia were implemented. A compact and fully automated microcoulometric sensor - actuator system for fast and long term stable enzymatic determinations was developed. The new measuring device allows nearly absolute determinations. Therefore the frequency of recalibration can be decreased considerably.