IN VITRO AND IN VIVO DETERMINATION OF DOPAMINE AND OTHER NEUROTRANSMITTERS USING CARBON FIBRE AND GLASSY CARBON ELECTRODES BY DIFFERENTIAL PULSE VOLTAMMETRY

Abstract

Electroanalysis of neurotransmitters using carbon fibre electrodes (CFE) is very important for the development of new drugs, for modern brain research and understanding of the molecular processes in the central nervous system. Essential fundamental works have been carried out by Adams [1], Gonon [2], Pons and Fleischmann [3] concerning pretreatment of the electrodes and techniques of measurement. However, there are still some questions, for instance the reproducibility of electrochemical and chemical pretreatment, influence of the kind of the fibre, of the application of polymer layers and of other modifications. Today commercial microelectrodes are still not avalaible. Users of electrodes usually have developed a method to be well applicable for themselves.