MONITORING AND CONTROL OF BIOTECHNOLOGICAL PRODUCTION PROCESSES BY BIO-FET-FIA-SENSORS

Abstract

Single and multisensor Field Effect Transistors FET with a pH-sensitive Si/SiO2/SizN4/TazOs-gate and reference electrode (for single semsor) were developed and used for manufacturing the following BIO-FETs: for glucose analysis: Glucose Oxidase-FETs (GOD-FET), for penicillin G and V analysis: Penicillin G Amidase- and Penicillinase-FETs, for urea analysis: Urease-FET, and for cephalosporin C analysis: Cephalosporinase-FET. The GOD-FETswereintegrated into the FIA-system of Eppendorf (EVA) and used for monitoring the glucose concentration in microbial cultivation and production processes with recombinant_Escherichia coli K12 MF, recombinant Escherichia coli JM103, Saccharomyces cerevisiae H620, and Candida boidinii. Urease-FET-FIA was used to monitor the urea concentration in a simulatedcultivation of Cephalosporium acremonium and UREASE-FET-FIA and GOD-FET-FIA for the monitoring of urea and glucose concentrations in simulated Saccharomyces cerevisiae cultivations.