CHARACTERISTICS AND APPLICATION OF A CONTINUOUS GLUCOSE REGISTRATION DEVICE USING THE MICRODIALYSIS TECHNIQUE

Abstract

A device for continuous registration of dissolved glucose was obtained by combining the microdialysis technique with the measuring flow chamber of a glucose monitor using the glucose oxidase method for the determination of glucose concentration. In in vitro experiments it was demonstrated that the glucose signal registered by this device is inversely related to the flow rate, which, in turn, is inversely related to the response time of the device. The glucose signal increased linearly with the area of the microdialysis working membrane and with the glucose concentrations of the standard solutions. The influences of temperature changes of the glucose standard solutions upon the glucose signal were determined by 1.32%/degc. In in vivo experiments, a needle type microdialysis probe was implanted subcutaneously in rats, whose blood glucose concentration was varied between 40 and 122 mg/dl. Blood glucose concentration was followed closely by the glucose signal obtained from the subcutaneous probe (r = 0.94) over a 240 min registration time period.