BIOCHEMICAL CHARACTERIZATION OFA HIGHLY SPECIFIC TRIMETHYLAMINE DEHYDROGENASE SUITED FOR THE APPLICATION IN BIOSENSORS
Abstract
A strain of Paracoccus possessing a NAD (P)*-independent trimethylamine dehydrogenase could be isolated by enrichment cultivation. The enzyme was purified up to 4.2 U/mg. Phenazine ethosulfate acts as an electron acceptor, other artificial mediators such as methylene blue or K3[Fe(CN)¢] are inactive. Important biochemical data are: pH-optimum: 9.0; substrates: specific for trimethylamine; dimethylamine, methylamine or $rimethylamine-N-oxide are not converted; Ky(trimethylamine): 6.6*¢10°° M. The calibration curve for trimethylamine (aqueous solution) using a kinetic photometric assay with dichlorophenol-indophenol shows a linearity between 0.1 - 0.8 uM trimethylamine.Citation
Biosensors : fundamentals, technologies and applications, 381 - 384Affiliation
Institut für Enzymtechnologie der Heinrich-Heine-Universität Düsseldorf; Forschungszentrum Jülich, Pf 2050, D-5170 Jülich; Kinderklinik der Heinrich-Heine-Universität Düsseldorf, Moorenstr. 5, D-4000 DüsseldorfType
Book chapterconference paper
Language
enSeries/Report no.
GBF monographs ; Volume 17ISSN
0930-4320ISBN
15608122063527284370
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