IMPORTANCE OF HETEROGENEOUS ELECTRON TRANSFER IN MONOENZYMATIC AND BIENZYMATIC ELECTROCHEMICAL SENSORS

Abstract

About several examples developed in the laboratory we discuss of the role of electronic transfer between electrode and ions or biomolecules in the increase of sensitivity and selectivity of biosensors. According to the kind of enzymes used in these biosensors it was possible to propose: - a monoenzymatic L.lactate electrode based on the direct electron transfer between platinum and a lactate dehydrogenase containing flavines and hemic groups; - bienzymatic electrodes for L.lactate, D.lactate and L.carnitine using both dehydrogenase and diaphorase and exploiting the principle of enzymatic amplification; - electrode for NADH using a double mediation with formate dehydrogenase and flavins.