BIOSENSOR SYSTEMSUTILIZING NADH AS AN INTERMEDIATE

Abstract

The combination of dehydrogenases and chemically modified graphite electrodes has proved particularly useful in the developmentof highly selective and sensitive biosensor systems. Amongthe applications given is a flow injection system for the determination of fructose with a linear range of 1 pM - 2 mM.As a means toincrease sensitivity further, substrate recycling was employed in the determination of ATP down to the nano-molar level. A creatine and creatinine assayillustrates the potential of coupled equilibria and coimmobilized enzymes. For the determination ofglucose in whole blood a dialyzer was integrated with a biosensor. The hematocrit dependence on the transfer of glucose through the dialysis membrane was studied.