NOVEL COVALENT AVIDIN IMMOBILIZATION ON GLASSY CARBON ELECTRODES USING A BIFUNCTIONAL REAGENT

Abstract

It is generally accepted that the stability and performance of a biosensor is determined by the way biocomponents are immobilized on the inorganic support. We present a covalent coupling method of the anchor protein avidin to a glassy carbon electrode surface using the heterobifunctional reagent m-maleimidobenzoyl-N-hydroxysuccinimide ester (MBS). Thus, a nearly complete, biologically active monolayer of avidin was obtained which is suitable for the immobilization of any biotinylated compound. These modified electrodes are potentially useful as amperometric biosensors.