ON-LINE MONITORING OF FAST BIOPROCESSES WITH FLOW INJECTION ANALYSIS AND GASCHROMATOGRAPHY

Abstract

For fundamental metabolic studies and effective control of bioprocesses on-line analysis of substrates, products and intermediates is required. For the investigation of the metabolism of B. subtilis on-line flow injection analysis for glucose has been applied together with a online capillary gaschromatograph for direct analysis of meso- and D/L 2.3-butanediol, acetoin and acetic acid in liquid samples in intervals of less than eight minutes.