Quantitative determination of cyclic diguanosine monophosphate concentrations in nucleotide extracts of bacteria by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry.
dc.contributor.author | Simm, Roger | |
dc.contributor.author | Morr, Michael | |
dc.contributor.author | Remminghorst, Uwe | |
dc.contributor.author | Andersson, Mats | |
dc.contributor.author | Römling, Ute | |
dc.date.accessioned | 2009-04-03T12:53:23Z | |
dc.date.available | 2009-04-03T12:53:23Z | |
dc.date.issued | 2009-03-01 | |
dc.identifier.citation | Quantitative determination of cyclic diguanosine monophosphate concentrations in nucleotide extracts of bacteria by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry. 2009, 386 (1):53-8 Anal. Biochem. | en |
dc.identifier.issn | 1096-0309 | |
dc.identifier.pmid | 19135022 | |
dc.identifier.doi | 10.1016/j.ab.2008.12.013 | |
dc.identifier.uri | http://hdl.handle.net/10033/64253 | |
dc.description.abstract | The physiological response to small molecules (secondary messengers) is the outcome of a delicate equilibrium between biosynthesis and degradation of the signal. Cyclic diguanosine monophosphate (c-di-GMP) is a novel secondary messenger present in many bacteria. It has a complex cellular metabolism whereby usually more than one enzyme synthesizing and degrading c-di-GMP is encoded by a bacterial genome. To assess the in vivo conditions of c-di-GMP signaling, we developed a high-performance liquid chromatography (HPLC)-mass spectrometry-based method to detect c-di-GMP with high sensitivity and to quantify the c-di-GMP concentration in the bacterial cell as described here in detail. We successfully used the methodology to determine and compare the c-di-GMP concentrations in bacterial species such as Salmonella typhimurium, Escherichia coli, Pseudomonas aeruginosa, and Vibrio cholerae. We describe the use of the methodology to assess the change in c-di-GMP concentration during the growth phase and the contribution of a point mutation in S. typhimurium to the overall cellular c-di-GMP concentration. | |
dc.language.iso | en | en |
dc.subject.mesh | Bacteria | en |
dc.subject.mesh | Cyclic GMP | en |
dc.subject.mesh | Methods | en |
dc.subject.mesh | Nucleotides | en |
dc.subject.mesh | Research Design | en |
dc.subject.mesh | Second Messenger Systems | en |
dc.subject.mesh | Tandem Mass Spectrometry | en |
dc.title | Quantitative determination of cyclic diguanosine monophosphate concentrations in nucleotide extracts of bacteria by matrix-assisted laser desorption/ionization-time-of-flight mass spectrometry. | en |
dc.type | Article | en |
dc.contributor.department | Department of Microbiology, Tumor and Cell Biology, Karolinska Institute, Stockholm, Sweden. | en |
dc.identifier.journal | Analytical biochemistry | en |
refterms.dateFOA | 2018-06-12T18:05:35Z | |
html.description.abstract | The physiological response to small molecules (secondary messengers) is the outcome of a delicate equilibrium between biosynthesis and degradation of the signal. Cyclic diguanosine monophosphate (c-di-GMP) is a novel secondary messenger present in many bacteria. It has a complex cellular metabolism whereby usually more than one enzyme synthesizing and degrading c-di-GMP is encoded by a bacterial genome. To assess the in vivo conditions of c-di-GMP signaling, we developed a high-performance liquid chromatography (HPLC)-mass spectrometry-based method to detect c-di-GMP with high sensitivity and to quantify the c-di-GMP concentration in the bacterial cell as described here in detail. We successfully used the methodology to determine and compare the c-di-GMP concentrations in bacterial species such as Salmonella typhimurium, Escherichia coli, Pseudomonas aeruginosa, and Vibrio cholerae. We describe the use of the methodology to assess the change in c-di-GMP concentration during the growth phase and the contribution of a point mutation in S. typhimurium to the overall cellular c-di-GMP concentration. |