• The metabolic response of P. putida KT2442 producing high levels of polyhydroxyalkanoate under single- and multiple-nutrient-limited growth: highlights from a multi-level omics approach.

      Poblete-Castro, Ignacio; Escapa, Isabel F; Jäger, Christian; Puchalka, Jacek; Lam, Carolyn Ming Chi; Schomburg, Dietmar; Prieto, María Auxiliadora; Martins dos Santos, Vítor A P; Helmholtz Centre for Infection Research (HZI), Braunschweig, Germany. ignacio.pobletecastro@helmholtz-hzi.de (2012)
      Pseudomonas putida KT2442 is a natural producer of polyhydroxyalkanoates (PHAs), which can substitute petroleum-based non-renewable plastics and form the basis for the production of tailor-made biopolymers. However, despite the substantial body of work on PHA production by P. putida strains, it is not yet clear how the bacterium re-arranges its whole metabolism when it senses the limitation of nitrogen and the excess of fatty acids as carbon source, to result in a large accumulation of PHAs within the cell. In the present study we investigated the metabolic response of KT2442 using a systems biology approach to highlight the differences between single- and multiple-nutrient-limited growth in chemostat cultures.