Isolation of isomangiferin from honeybush (Cyclopia subternata) using high-speed counter-current chromatography and high-performance liquid chromatography.

de Beer, Dalene; Jerz, Gerold; Joubert, Elizabeth; Wray, Victor; Winterhalter, Peter

dc.contributor.author	de Beer, Dalene
dc.contributor.author	Jerz, Gerold
dc.contributor.author	Joubert, Elizabeth
dc.contributor.author	Wray, Victor
dc.contributor.author	Winterhalter, Peter
dc.date.accessioned	2009-06-23T08:26:46Z
dc.date.available	2009-06-23T08:26:46Z
dc.date.issued	2009-05-08
dc.identifier.citation	Isolation of isomangiferin from honeybush (Cyclopia subternata) using high-speed counter-current chromatography and high-performance liquid chromatography. 2009, 1216 (19):4282-9 J Chromatogr A	en
dc.identifier.issn	1873-3778
dc.identifier.pmid	19272608
dc.identifier.doi	10.1016/j.chroma.2009.02.056
dc.identifier.uri	http://hdl.handle.net/10033/71233
dc.description.abstract	Isomangiferin was isolated from Cyclopia subternata using a multi-step process including extraction, liquid-liquid partitioning, high-speed counter-current chromatography (HSCCC) and semi-preparative reversed-phase high-performance liquid chromatography (HPLC). Enrichment of phenolic compounds in a methanol extract of C. subternata leaves was conducted using liquid-liquid partitioning with ethyl acetate-methanol-water (1:1:2, v/v). The enriched fraction was further fractionated using HSCCC with a ternary solvent system consisting of tert-butyl methyl ether-n-butanol-acetonitrile-water (3:1:1:5, v/v). Isomangiferin was isolated by semi-preparative reversed-phase HPLC from a fraction containing mostly mangiferin and isomangiferin. The chemical structure of isomangiferin was confirmed by LC-high-resolution electrospray ionization MS, as well as one- and two-dimensional NMR spectroscopy.
dc.language.iso	en	en
dc.title	Isolation of isomangiferin from honeybush (Cyclopia subternata) using high-speed counter-current chromatography and high-performance liquid chromatography.	en
dc.type	Article	en
dc.contributor.department	ARC Infruitec-Nietvoorbij, Stellenbosch, South Africa. dbeerd@arc.agric.za	en
dc.identifier.journal	Journal of chromatography. A	en
refterms.dateFOA	2018-06-12T23:51:00Z
html.description.abstract	Isomangiferin was isolated from Cyclopia subternata using a multi-step process including extraction, liquid-liquid partitioning, high-speed counter-current chromatography (HSCCC) and semi-preparative reversed-phase high-performance liquid chromatography (HPLC). Enrichment of phenolic compounds in a methanol extract of C. subternata leaves was conducted using liquid-liquid partitioning with ethyl acetate-methanol-water (1:1:2, v/v). The enriched fraction was further fractionated using HSCCC with a ternary solvent system consisting of tert-butyl methyl ether-n-butanol-acetonitrile-water (3:1:1:5, v/v). Isomangiferin was isolated by semi-preparative reversed-phase HPLC from a fraction containing mostly mangiferin and isomangiferin. The chemical structure of isomangiferin was confirmed by LC-high-resolution electrospray ionization MS, as well as one- and two-dimensional NMR spectroscopy.