Genomewide identification of genetic determinants of antimicrobial drug resistance in Pseudomonas aeruginosa.
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractThe emergence of antimicrobial drug resistance is of enormous public concern due to the increased risk of delayed treatment of infections, the increased length of hospital stays, the substantial increase in the cost of care, and the high risk of fatal outcomes. A prerequisite for the development of effective therapy alternatives is a detailed understanding of the diversity of bacterial mechanisms that underlie drug resistance, especially for problematic gram-negative bacteria such as Pseudomonas aeruginosa. This pathogen has impressive chromosomally encoded mechanisms of intrinsic resistance, as well as the potential to mutate, gaining resistance to current antibiotics. In this study we have screened the comprehensive nonredundant Harvard PA14 library for P. aeruginosa mutants that exhibited either increased or decreased resistance against 19 antibiotics commonly used in the clinic. This approach identified several genes whose inactivation sensitized the bacteria to a broad spectrum of different antimicrobials and uncovered novel genetic determinants of resistance to various classes of antibiotics. Knowledge of the enhancement of bacterial susceptibility to existing antibiotics and of novel resistance markers or modifiers of resistance expression may lay the foundation for effective therapy alternatives and will be the basis for the development of new strategies in the control of problematic multiresistant gram-negative bacteria.
CitationGenomewide identification of genetic determinants of antimicrobial drug resistance in Pseudomonas aeruginosa. 2009, 53 (6):2522-31 Antimicrob. Agents Chemother.
AffiliationChronic Pseudomonas Infections Research Group, Helmholtz Center for Infection Research, Inhoffenstrasse 7, D-38124 Braunschweig, Germany.
The following license files are associated with this item:
- Novel genetic determinants of low-level aminoglycoside resistance in Pseudomonas aeruginosa.
- Authors: Schurek KN, Marr AK, Taylor PK, Wiegand I, Semenec L, Khaira BK, Hancock RE
- Issue date: 2008 Dec
- [Screen and identification of genes involved in multidrug resistance in Pseudomonas aeruginosa].
- Authors: Zhao X, Liang H, Shen L, Dong Z, Duan K
- Issue date: 2008 Aug
- Efflux as a mechanism of resistance to antimicrobials in Pseudomonas aeruginosa and related bacteria: unanswered questions.
- Authors: Schweizer HP
- Issue date: 2003 Mar 31
- Role of efflux mechanisms on fluoroquinolone resistance in Streptococcus pneumoniae and Pseudomonas aeruginosa.
- Authors: Zhanel GG, Hoban DJ, Schurek K, Karlowsky JA
- Issue date: 2004 Dec
- Pseudomonas aeruginosa reveals high intrinsic resistance to penem antibiotics: penem resistance mechanisms and their interplay.
- Authors: Okamoto K, Gotoh N, Nishino T
- Issue date: 2001 Jul