Cationic hydrous thorium dioxide colloids – a useful tool for staining negatively charged surface matrices of bacteria for use in energy-filtered transmission electron microscopy
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractBackground Synthesis of cationic hydrous thorium dioxide colloids (ca. 1.0 to 1.7 nm) has been originally described by Müller  and Groot  and these have been used by Groot to stain acidic glucosaminoglycans for ultrastructure research of different tissues by conventional transmission electron microscopy. Results Synthesis of colloidal thorium dioxide has been modified and its use as a suitable stain of acidic mucopolysaccharides and other anionic biopolymers from bacteria, either as whole mount preparations or as preembedment labels, is described. The differences in stain behavior relative to commonly used rutheniumred-lysine and Alcian Blue™ electron dense acidic stains has been investigated and its use is exemplified for Pseudomonas aeruginosa adjacent cell wall biopolymers. For the first time thorificated biopolymers, i.e. bacterial outer cell wall layers, have been analysed at the ultrastructural level with electron energy loss spectroscopy (EELS) and electron spectroscopic imaging (ESI), leading to excellent contrast and signal strength for these extracellular biopolymers. Conclusion Application of cationic hydrous ThO2 colloids for tracing acidic groups of the bacterial surface and/or EPS has been shown to be rather effective by transmission electron microscopy. Because of its high electron density and its good diffusibility it stains and outlines electro-negative charges within these biopolymers. In combination with ESI, based on integrated energy-filtered electron microscopy (EFTEM) Th-densities and thus negative charge densities can be discriminated from other elemental densities, especially in environmental samples, such as biofilms.
CitationBMC Microbiology 2006 6:59
PubMed Central ID1524781
The following license files are associated with this item:
- Visualization of the glomerular endothelial glycocalyx by electron microscopy using cationic colloidal thorium dioxide.
- Authors: Hegermann J, Lünsdorf H, Ochs M, Haller H
- Issue date: 2016 Jan
- Ultrastructural and electron energy-loss spectroscopic analysis of an extracellular filamentous matrix of an environmental bacterial isolate.
- Authors: Böckelmann U, Lünsdorf H, Szewzyk U
- Issue date: 2007 Sep
- Electron energy-loss spectroscopy as a tool for elemental analysis in biological specimens.
- Authors: Kapp N, Studer D, Gehr P, Geiser M
- Issue date: 2007
- Energy-filtered transmission electron microscopy of biological samples on highly transparent carbon nanomembranes.
- Authors: Rhinow D, Büenfeld M, Weber NE, Beyer A, Gölzhäuser A, Kühlbrandt W, Hampp N, Turchanin A
- Issue date: 2011 Apr
- Enhanced visualization of microbial biofilms by staining and environmental scanning electron microscopy.
- Authors: Priester JH, Horst AM, Van de Werfhorst LC, Saleta JL, Mertes LA, Holden PA
- Issue date: 2007 Mar