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dc.contributor.authorMeidtner, Karina
dc.contributor.authorSchwarzenbacher, Hermann
dc.contributor.authorScharfe, Maren
dc.contributor.authorSeveritt, Simone
dc.contributor.authorBlöcker, Helmut
dc.contributor.authorFries, Ruedi
dc.date.accessioned2010-03-12T09:52:44Z
dc.date.available2010-03-12T09:52:44Z
dc.date.issued2009
dc.identifier.citationHaplotypes of the porcine peroxisome proliferator-activated receptor delta gene are associated with backfat thickness. 2009, 10:76 BMC Genet.en
dc.identifier.issn1471-2156
dc.identifier.pmid19943979
dc.identifier.doi10.1186/1471-2156-10-76
dc.identifier.urihttp://hdl.handle.net/10033/94173
dc.description.abstractBACKGROUND: Peroxisome proliferator-activated receptor delta belongs to the nuclear receptor superfamily of ligand-inducible transcription factors. It is a key regulator of lipid metabolism. The peroxisome proliferator-activated receptor delta gene (PPARD) has been assigned to a region on porcine chromosome 7, which harbours a quantitative trait locus for backfat. Thus, PPARD is considered a functional and positional candidate gene for backfat thickness. The purpose of this study was to test this candidate gene hypothesis in a cross of breeds that were highly divergent in lipid deposition characteristics. RESULTS: Screening for genetic variation in porcine PPARD revealed only silent mutations. Nevertheless, significant associations between PPARD haplotypes and backfat thickness were observed in the F2 generation of the Mangalitsa x Piétrain cross as well as a commercial German Landrace population. Haplotype 5 is associated with increased backfat in F2 Mangalitsa x Piétrain pigs, whereas haplotype 4 is associated with lower backfat thickness in the German Landrace population. Haplotype 4 and 5 carry the same alleles at all but one SNP. Interestingly, the opposite effects of PPARD haplotypes 4 and 5 on backfat thickness are reflected by opposite effects of these two haplotypes on PPAR-delta mRNA levels. Haplotype 4 significantly increases PPAR-delta mRNA levels, whereas haplotype 5 decreases mRNA levels of PPAR-delta. CONCLUSION: This study provides evidence for an association between PPARD and backfat thickness. The association is substantiated by mRNA quantification. Further studies are required to clarify, whether the observed associations are caused by PPARD or are the result of linkage disequilibrium with a causal variant in a neighbouring gene.
dc.language.isoenen
dc.subject.meshAdipose Tissueen
dc.subject.meshAnimalsen
dc.subject.meshHaplotypesen
dc.subject.meshPPAR deltaen
dc.subject.meshRNA, Messengeren
dc.subject.meshSwineen
dc.titleHaplotypes of the porcine peroxisome proliferator-activated receptor delta gene are associated with backfat thickness.en
dc.typeArticleen
dc.contributor.departmentChair of Animal Breeding, Technical University of Munich, Hochfeldweg 1, 85354 Freising - Weihenstephan, Germany. karina.meidtner@tierzucht.tum.deen
dc.identifier.journalBMC geneticsen
refterms.dateFOA2018-06-12T17:20:54Z
html.description.abstractBACKGROUND: Peroxisome proliferator-activated receptor delta belongs to the nuclear receptor superfamily of ligand-inducible transcription factors. It is a key regulator of lipid metabolism. The peroxisome proliferator-activated receptor delta gene (PPARD) has been assigned to a region on porcine chromosome 7, which harbours a quantitative trait locus for backfat. Thus, PPARD is considered a functional and positional candidate gene for backfat thickness. The purpose of this study was to test this candidate gene hypothesis in a cross of breeds that were highly divergent in lipid deposition characteristics. RESULTS: Screening for genetic variation in porcine PPARD revealed only silent mutations. Nevertheless, significant associations between PPARD haplotypes and backfat thickness were observed in the F2 generation of the Mangalitsa x Piétrain cross as well as a commercial German Landrace population. Haplotype 5 is associated with increased backfat in F2 Mangalitsa x Piétrain pigs, whereas haplotype 4 is associated with lower backfat thickness in the German Landrace population. Haplotype 4 and 5 carry the same alleles at all but one SNP. Interestingly, the opposite effects of PPARD haplotypes 4 and 5 on backfat thickness are reflected by opposite effects of these two haplotypes on PPAR-delta mRNA levels. Haplotype 4 significantly increases PPAR-delta mRNA levels, whereas haplotype 5 decreases mRNA levels of PPAR-delta. CONCLUSION: This study provides evidence for an association between PPARD and backfat thickness. The association is substantiated by mRNA quantification. Further studies are required to clarify, whether the observed associations are caused by PPARD or are the result of linkage disequilibrium with a causal variant in a neighbouring gene.


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