Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Your vote was cast
Thank you for your feedback
Thank you for your feedback
MetadataShow full item record
AbstractIsoprene is a volatile and climate-altering hydrocarbon with an atmospheric concentration similar to that of methane. It is well established that marine algae produce isoprene; however, until now there was no specific information about marine isoprene sinks. Here we demonstrate isoprene consumption in samples from temperate and tropical marine and coastal environments, and furthermore show that the most rapid degradation of isoprene coincides with the highest rates of isoprene production in estuarine sediments. Isoprene-degrading enrichment cultures, analysed by denaturing gradient gel electrophoresis and 454 pyrosequencing of the 16S rRNA gene and by culturing, were generally dominated by Actinobacteria, but included other groups such as Alphaproteobacteria and Bacteroidetes, previously not known to degrade isoprene. In contrast to specialist methane-oxidizing bacteria, cultivated isoprene degraders were nutritionally versatile, and nearly all of them were able to use n-alkanes as a source of carbon and energy. We therefore tested and showed that the ubiquitous marine hydrocarbon-degrader, Alcanivorax borkumensis, could also degrade isoprene. A mixture of the isolates consumed isoprene emitted from algal cultures, confirming that isoprene can be metabolized at low, environmentally relevant concentrations, and suggesting that, in the absence of spilled petroleum hydrocarbons, algal production of isoprene could maintain viable populations of hydrocarbon-degrading microbes. This discovery of a missing marine sink for isoprene is the first step in obtaining more robust predictions of its flux, and suggests that algal-derived isoprene provides an additional source of carbon for diverse microbes in the oceans.
CitationCharacterization of marine isoprene-degrading communities. 2009, 11 (12):3280-91 Environ. Microbiol.
AffiliationDepartment of Biological Sciences, University of Essex, Wivenhoe Park, Colchester, CO4 3SQ, UK.
The following license files are associated with this item:
- Identification and characterisation of isoprene-degrading bacteria in an estuarine environment.
- Authors: Johnston A, Crombie AT, El Khawand M, Sims L, Whited GM, McGenity TJ, Colin Murrell J
- Issue date: 2017 Sep
- Biotechnological potential for degradation of isoprene: a review.
- Authors: Srivastva N, Singh A, Bhardwaj Y, Dubey SK
- Issue date: 2018 Jun
- Microbial cycling of isoprene, the most abundantly produced biological volatile organic compound on Earth.
- Authors: McGenity TJ, Crombie AT, Murrell JC
- Issue date: 2018 Apr
- Effects of temperature and biostimulation on oil-degrading microbial communities in temperate estuarine waters.
- Authors: Coulon F, McKew BA, Osborn AM, McGenity TJ, Timmis KN
- Issue date: 2007 Jan
- Oceanobacter-related bacteria are important for the degradation of petroleum aliphatic hydrocarbons in the tropical marine environment.
- Authors: Teramoto M, Suzuki M, Okazaki F, Hatmanti A, Harayama S
- Issue date: 2009 Oct