Intrinsic thermal sensing controls proteolysis of Yersinia virulence regulator RovA.
Name:
Herbst et al_final.pdf
Size:
2.000Mb
Format:
PDF
Description:
Original Open Access article
Average rating
Cast your vote
You can rate an item by clicking the amount of stars they wish to award to this item.
When enough users have cast their vote on this item, the average rating will also be shown.
Star rating
Your vote was cast
Thank you for your feedback
Thank you for your feedback
Authors
Herbst, KatharinaBujara, Matthias
Heroven, Ann Kathrin
Opitz, Wiebke
Weichert, Martin
Zimmermann, Ariane
Dersch, Petra
Issue Date
2009-05
Metadata
Show full item recordAbstract
Pathogens, which alternate between environmental reservoirs and a mammalian host, frequently use thermal sensing devices to adjust virulence gene expression. Here, we identify the Yersinia virulence regulator RovA as a protein thermometer. Thermal shifts encountered upon host entry lead to a reversible conformational change of the autoactivator, which reduces its DNA-binding functions and renders it more susceptible for proteolysis. Cooperative binding of RovA to its target promoters is significantly reduced at 37 degrees C, indicating that temperature control of rovA transcription is primarily based on the autoregulatory loop. Thermally induced reduction of DNA-binding is accompanied by an enhanced degradation of RovA, primarily by the Lon protease. This process is also subject to growth phase control. Studies with modified/chimeric RovA proteins indicate that amino acid residues in the vicinity of the central DNA-binding domain are important for proteolytic susceptibility. Our results establish RovA as an intrinsic temperature-sensing protein in which thermally induced conformational changes interfere with DNA-binding capacity, and secondarily render RovA susceptible to proteolytic degradation.Citation
Intrinsic thermal sensing controls proteolysis of Yersinia virulence regulator RovA. 2009, 5 (5):e1000435 PLoS Pathog.Affiliation
Institut für Mikrobiologie, Technische Universität Braunschweig, Germany.Journal
PLoS pathogensPubMed ID
19468295Type
ArticleLanguage
enISSN
1553-7374ae974a485f413a2113503eed53cd6c53
10.1371/journal.ppat.1000435
Scopus Count
The following license files are associated with this item:
Related articles
- Structural basis for intrinsic thermosensing by the master virulence regulator RovA of Yersinia.
- Authors: Quade N, Mendonca C, Herbst K, Heroven AK, Ritter C, Heinz DW, Dersch P
- Issue date: 2012 Oct 19
- RovM, a novel LysR-type regulator of the virulence activator gene rovA, controls cell invasion, virulence and motility of Yersinia pseudotuberculosis.
- Authors: Heroven AK, Dersch P
- Issue date: 2006 Dec
- Analysis of RovA, a transcriptional regulator of Yersinia pseudotuberculosis virulence that acts through antirepression and direct transcriptional activation.
- Authors: Tran HJ, Heroven AK, Winkler L, Spreter T, Beatrix B, Dersch P
- Issue date: 2005 Dec 23
- Regulatory elements implicated in the environmental control of invasin expression in enteropathogenic Yersinia.
- Authors: Heroven AK, Böhme K, Tran-Winkler H, Dersch P
- Issue date: 2007
- RovA, a global regulator of Yersinia pestis, specifically required for bubonic plague.
- Authors: Cathelyn JS, Crosby SD, Lathem WW, Goldman WE, Miller VL
- Issue date: 2006 Sep 5